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KMID : 1377020200170030387
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Tissue Engineering and Regenerative Medicine
2020 Volume.17 No. 3 p.387 ~ p.399
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Evaluation of Collagen Gel-Associated Human Nasal Septum-Derived Chondrocytes As a Clinically Applicable Injectable Therapeutic Agent for Cartilage Repair
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Lim Mi-Hyun
Jeun Jung-Ho
Kim Do-Hyun
Park Sun-Hwa
Kim Seok-Jung
Lee Weon-Sun
Hwang Se-Hwan
Lim Jung-Yeon
Kim Sung-Won
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Abstract
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Background: Articular cartilage injury has a poor repair ability and limited regeneration capacity with therapy based on articular chondrocytes (ACs) implantation. Here, we validated the hypothesis that human nasal septum-derived chondrocytes (hNCs) are potent therapeutic agents for clinical use in cartilage tissue engineering using an injectable hydrogel, type I collagen (COL1).
Methods: We manufactured hNCs incorporated in clinical-grade soluble COL1 and investigated their clinical potential as agents in an articular defect model.
Results: The hNCs encapsulated in COL1 (hNC-collagen) were uniformly distributed throughout the collagen and showed much greater growth rate than hACs encapsulated in collagen for the 14 days of culture. Fluorescent staining of hNC-collagen showed high expression levels of chondrocyte-specific proteins under clinical conditions. Moreover, a negative mycoplasma screening result were obtained in culture of hNC-collagen. Notably, implantation of hNC-collagen increased the repair of osteochondral defects in rats compared with implantation of collagen only. Many human cells were detected within the cartilage defects.
Conclusion: These results provide reliable evidences supporting for clinical applications of hNC-collagen in regenerative medicine for cartilage repair.
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KEYWORD
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Cartilage regeneration, Chondrocytes, Human nasal septum, Soluble type I collagen, Tissue engineering
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